Diagnosis: Male factor infertility complicated by DH's Robertsonian Translocation 13;15.
IVF #1 July 2006 (Pensacola, FL) 23 eggs retrieved. 18 fertilized via ICSI. PGD. 8 "normal" embryos. Freeze all cycle due to severe OHSS. Hospitalized for 8 days.
FET #1 November 2006 (Pensacola, FL) Canceled at the last minute (after taking all the meds and shots for about 3 weeks) due to nonsuppression.
FET #2 September 2007 (Pensacola, FL) Transferred 1 female embryo. BFN.
FET #3 November 2007 (Pensacola, FL) Transferred 2 male embryos. BFN.
Decided to change fertlility clinics, so consulted with CCRM in Denver and SIRM in Las Vegas. Chose CCRM and transferred remaining 5 frozen embryos from Pensacola to Denver to have them genetically re-checked. 3 embryos died upon thaw, 1 embryo's genetic test came back inconclusive. 1 embryo normal for chromosomes 13 & 15. Re-froze the one normal embryo and the one inconclusive embryo.
One-day work up at CCRM in April 2008. FSH 7.18, E2 29, AMH 4.3, AFC 35+, genetic testing on me all came back normal. DH's genetic testing came back with 65% of sperm are affected with the unbalanced translocation.
IVF #2 July 2008 (Denver, CO) 30 eggs retrieved. 26 mature. 23 fertilized via ICSI. PGD. 7 embryos normal for chromosomes 13 & 15. 1 embryo made it to freeze. Transferred 2 grade AA blasts. BFN.
IVF #3 November 2008 (Denver, CO) 34 eggs retrieved. 25 mature. 22 fertilized via ICSI. 15 blasts for CGH testing. Results: 8 abnormal & 7 no results. The 7 no results will be thawed, re-biopsied, and re-vitrified; and the cells will be sent for FISH analysis for the translocation. Should get those results by Christmas. UPDATE: 1 blast is normal for 13 & 15 and 1 blast still no result.
FET #4 February 2009 (Denver, CO) Our first ever BFP!! Beta #1 (9dp5dt): 174 !!!!! Beta #2 (11dp5dt): 401 !!!!!
To re-cap, in November 2008 we had the cells from 15 blasts sent for CGH analysis. There were 8 that came back abnormal and 7 with no results. The 7 NR embryos were biopsied again and sent for FISH testing (13, 15, & 21). Only 1 came back normal. We didn't have any abnormalities with 21, but I wanted it screened anyway. The embryos that came back abnormal had something wrong with either 13, 15, or both.
So we have in the freezer three frozen blasts normal for chromosomes 13 & 15 and two no results (even after 2 different biopsies). The first normal blast is from the July 2006 IVF/ICSI/PGD-FISH cycle here in Pensacola. It's been through a lot, so we're not sure if it will survive the thaw. It went through its first biopsy (FISH 13, 18, 21, & sex chromosomes) on Day 3 here in Pensacola. Then it was grown to the blast stage and frozen the old-fashioned way (slow freezing process where the embryologist had to draw out fluid before freezing). The Pensacola RE didn't screen for chromosome 15, so we couldn't trust the FISH results, as we know that there is a known genetic issue with both 13 & 15. After almost 2 years in storage at the Pensacola RE's clinic, it was FedEx'ed to CCRM in a nitrogen container. Then it was thawed, re-biopsied (for FISH analysis #2 - 13 & 15), and re-frozen (via vitrification). So that blast is normal for 13, 15, 18, 21, and sex chromosomes. The second normal (FISH 13 & 15 only) blast is from the July 2008 IVF/ICSI/PGD-FISH cycle at CCRM. It only went through one biopsy (Day 3) and then vitrified on Day 5. Our third normal (for 13, 15, & 21) blast is from our latest cycle - the November 2008 IVF/ICSI/CGH/PGD-FISH cycle. It went through its first biopsy (Day 5 trophectoderm biopsy) for CGH (no result), vitrified, thawed, second trophectoderm biopsy (for FISH 13, 15, & 21), and then re-vitrified.
After 3 IVFs, we have 3 'normal' and 2 'no results' frozen blasts. I am saddened that after all we've gone through ... 3 IVFs (PGD-FISH & CGH), 3 FETs, ~190 injections, 1 8-day hospitalization (due to OHSS), 1 HSG, 2 hysteroscopies, 1 endometrial biopsy, 2 DNA karyotypes, Cystic Fibrosis screening, Fragile X screening, 1 transrectal ultrasound (DH is a trooper), ~35 dates with the dildo-cam, countless blood tests, 87 eggs retrieved, 69 mature eggs, 63 embryos that were created... now we have only 5 to work with. I am sure that we will be punished for that one day when we have "the talk" with God. But I am so desperate right now to have our genetic child(ren) that I am willing to risk extra time in purification in Purgatory or even going to Hell.